5. 18–8 Stainless-steel socket head screw, M3 0.5 mm thread,
6 mm long for bracket and motor holding.
6. Super-corrosion-resistant flat-tip set screws, 316 stainless steel,
10–32 thread, 3/1600 long for shaft holder.
7. Four washers.
8. Teflon tape for pipe threads to ensure water-tight threads.
9. Sterile non-absorbable surgical silk suture #0–0 (2 20 cm).
10. Two polypropylene plastic narrow cable ties 400 Long.
11. Two polypropylene plastic standard cable ties 600 Long.
12. Three 125 mL sterile Erlenmeyer flasks with 2-hole rubber
stopper.
13. One 250 mL sterile Erlenmeyer flask with 2-hole rubber
stopper.
14. Three 500 mL Duran bottles for detergents and waste.
15. Five sterile hybridization bottles (for the tracheas).
16. USB temperature sensor (1/800 NPT stainless-steel housing
with a 100 long probe).
17. Five 50 mL plastic pipettes.
18. Sterile cotton tip applicator.
2.4
Tools
1. Nine-piece metric hex L-key set.
2. Slip-joint pliers.
3. Flathead screwdriver.
2.5
Devices
1. 12 V 5 A switching power supply adapter.
2. 6 rpm precision spur gear motor.
3. Arduino 4-channel motor shield–1.2 A.
4. Electric coaxial cable.
5. Ismatec®
Reglo
3-independent
channel
control
peristaltic pump.
2.6
Antibiotics,
Media, and Detergents
1. Imipenem/Cilastatin 500 mg/500 mg.
2. Ceftazidime 1 g.
3. Colistimethate 150 mg.
4. Fluconazole 400 mg/200 mL.
5. Cell culture grade water.
6. Dulbecco’s
Modified
Eagle’s
medium
high
glucose
L-
glutamine.
7. Fetal bovine serum.
8. Gibco™Antibiotic-Antimycotic (100).
170
Alba E. Marin-Araujo et al.